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KMID : 0357319800150010071
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Journal of the Korean Society for Microbiology
1980 Volume.15 No. 1 p.71 ~ p.75
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Purification of Anti-ox Red Blood Cell IgG Antibody for T subset Assay
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夏潤文/Ha, Youn-Mun
李振鏞/林壽德/Lee, Jean-Yong/Lim, Soo-Duk
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Abstract
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Antisera to ox red blood cell were. prepared by intraperitoneal hyper multiple injections without adjuvant in outbred white rabbits. Purified IgG fraction from these rabbits anti-ox red blood cell antiserum for T subset assay vas obtained by precipitation with 50% saturated ammonium sulphate followed by DEAEcellulose chromatography and Sephadex G-i30 gel filtration. These purified IgG fraction was compared -with Cappel.company standard IgG fraction for Ta subpopulation assay.
We used home-made IgG fraction and obtained favorable results in To subpopulations assay as Cappel company standard IgG fraction.
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